Free Testosterone Direct: Normal Range by Age in Men
Other names: Free Testosterone(Direct), Free Testosterone, Direct, Free Testosterone Direct, Free Testost Direct, Testosterone, Free, Direct, Free Testosterone Direct LC, Fr Testosterone Serum LC, Fr Testost Dir, Free Direct Testosterone, Testosterone Free Direct, Free Test Direct, Testost Free Serpl-MCNC
Optimal Result:
8.7 - 25.1 pg/mL.
AT A GLANCE
- Free testosterone (direct) measures the small fraction of testosterone in your blood that is not bound to proteins — the portion your body can actually use immediately
- Normal range for men aged 30–39: 8.7–25.1 pg/mL (LabCorp direct assay); ranges vary by age and lab method
- Levels decline predictably with age — a result that is low for a 35-year-old may be normal for a 65-year-old; always compare to the age-matched reference range on your report
- "Low free testosterone direct" is one of the most searched result interpretations — if your result is flagged low, this page covers what that means, why it happens, and what comes next
- "Free testosterone direct" is a specific measurement method — it is different from "calculated free testosterone," which is derived from a formula using total testosterone and SHBG
- LabCorp reports this test as "Free Testosterone, Direct" or "Free Testosterone Direct LC" — Quest reports it differently; reference ranges between labs are not directly interchangeable
- Low free testosterone with normal total testosterone is a clinically important pattern — usually caused by elevated SHBG — and is covered in its own section below
What Is Free Testosterone (Direct)?
Most testosterone in the blood is bound to proteins — approximately 44% to sex hormone-binding globulin (SHBG) and 54% to albumin. Only about 1–3% circulates unbound. This unbound fraction is called free testosterone. It is the biologically active form that can enter cells and exert effects on muscle, bone, libido, mood, cognition, and energy without requiring release from a carrier protein first.
Free testosterone (direct) measures this unbound fraction directly, using an immunoassay or — in higher-quality testing — equilibrium dialysis or ultrafiltration followed by mass spectrometry. The word "direct" distinguishes it from calculated free testosterone, which is not measured at all but rather estimated from a mathematical formula using total testosterone and SHBG levels.
Why free testosterone matters more than total testosterone in some situations:
Total testosterone can appear normal even when the biologically active fraction is critically low — particularly in men with elevated SHBG. SHBG binds testosterone tightly, removing it from circulation. A man with high SHBG may have a "normal" total testosterone on paper but very little free testosterone available to his tissues. In this scenario, free testosterone (direct) is the more clinically meaningful number.
What Does Your Free Testosterone Result Mean?
Find your result in the table below, then read the age-specific context underneath.
| Result (pg/mL) | Men aged 20–39 | Men aged 40–59 | Men aged 60+ |
|---|---|---|---|
| Below 5 | Very low — warrants evaluation | Very low — warrants evaluation | Below normal |
| 5–7 | Below normal range | Low-normal to borderline | Low-normal |
| 7–9 | Borderline low | Low-normal | Within range |
| 9–15 | Mid-to-lower normal | Normal range | Normal to optimal |
| 15–25 | Healthy mid-to-upper range | Upper-normal range | Above typical range |
| Above 25 | Upper range — evaluate if symptomatic | Elevated for age | Elevated for age |
| Above 30 | Above reference range | Above reference range | Above reference range |
Common specific results and what they mean:
3.9 pg/mL — Very low for any adult age group. Below the lower reference limit for men over 60 (6.6 pg/mL) and substantially below the limit for men under 50. Warrants clinical evaluation regardless of symptoms.
7.3 pg/mL — Below the lower reference limit for men aged 30–39 (8.7 pg/mL) and 20–29 (9.3 pg/mL). Within range for men over 60. In a man aged 30–50, this result with symptoms is clinically significant.
8.9 pg/mL — Within the lower portion of the normal range for men aged 30–39 (8.7–25.1 pg/mL). Borderline for men aged 20–29. If symptomatic, worth discussing with a doctor even if not flagged "low."
12 pg/mL — Mid-to-lower normal for men aged 30–49. Clinically unremarkable if asymptomatic. If symptoms of low testosterone are present alongside this result, SHBG should be checked.
15 pg/mL — Solidly within normal range for all adult age groups. No clinical concern on this number alone.
22 pg/mL — Upper-normal for most age groups. Normal for men aged 20–39; upper end or above reference for men over 50.
Free Testosterone Age Decline Visualization
Source: LabCorp direct analog immunoassay (test #140103). Values in pg/mL.
Free Testosterone Direct vs. Calculated Free Testosterone: What's the Difference?
This is one of the most searched questions connected to this result — and the answer has real clinical implications.
Free Testosterone Direct — the laboratory physically measures the unbound testosterone in your blood sample, typically using equilibrium dialysis (the gold standard) or a direct analog immunoassay. This produces an actual measured value.
Calculated Free Testosterone — no measurement is taken. Instead, a formula (most commonly the Vermeulen equation) uses your total testosterone, SHBG, and albumin levels to estimate what the free fraction should be. This appears on some reports as "testosterone free, calc" or "calc free testosterone."
Which is more accurate? The equilibrium dialysis method is considered the gold standard and most accurate. Direct analog immunoassays — the most common "direct" method at commercial labs including LabCorp — are faster and less expensive but have known limitations; they can underestimate free testosterone and are sensitive to assay variability. Calculated free testosterone using the Vermeulen equation is actually considered more reliable than direct analog immunoassay by some endocrinology guidelines, provided total testosterone and SHBG were measured accurately.
In practice: if your result says "free testosterone, direct" from LabCorp or Quest, it was measured by direct immunoassay. If it says "calc free testosterone" or "free testosterone, calculated," it was estimated. Both are clinically useful; neither is perfect. Use our free testosterone calculator to estimate your Vermeulen free T from your total testosterone, SHBG, and albumin — and compare it directly against your direct assay result.
Why Endocrinologists Debate Free Testosterone Testing Methods
Free testosterone is one of the most technically difficult hormones to measure accurately, and the method your lab used matters more than most patients realize.
Equilibrium dialysis is the gold standard. The blood sample is dialyzed through a semipermeable membrane to physically separate the free fraction, which is then measured directly. It is highly accurate but slow, expensive, and available only at specialized reference labs. LabCorp offers it under test code 143575. The Endocrine Society recommends equilibrium dialysis when precise free testosterone measurement is clinically critical.
Direct analog immunoassay is what most commercial labs use — including LabCorp (test #140103) and Quest Diagnostics (test #15983). It is faster and far less expensive, which is why it dominates routine testing. However, multiple studies have documented that direct analog assays systematically underestimate free testosterone and show poor agreement with equilibrium dialysis results. The Endocrine Society's 2018 Clinical Practice Guideline explicitly states that direct analog immunoassay is not a reliable measure of free testosterone. Despite this, it remains the most widely ordered method in the US because of cost and turnaround constraints.
Calculated free testosterone using the Vermeulen equation produces an estimate rather than a measurement — but counterintuitively, many endocrinologists consider a well-performed Vermeulen calculation more reliable than a direct analog immunoassay result, provided that total testosterone and SHBG were accurately measured. This is why some specialist reports will show a calculated value alongside or instead of a direct measurement.
What this means for interpreting your result: if your lab report says "free testosterone, direct" from LabCorp or Quest, it was measured by direct analog immunoassay. The number is clinically useful for detecting clear deficiency or elevation, but may not be as precise as equilibrium dialysis or a validated calculated value. If your result seems inconsistent with your symptoms — particularly if you feel symptomatic but your result is borderline normal — asking your doctor to also measure SHBG and calculate free testosterone using the Vermeulen equation is a reasonable and well-supported next step. The calculator on this page uses the Vermeulen equation if you want to compare your calculated value to your direct assay result.
Normal Range by Age — Free Testosterone Direct (Men)
Free testosterone declines progressively with age in men. Using the LabCorp direct assay reference ranges (the most common source for US lab reports):
| Age | Normal range (pg/mL) | Notes |
|---|---|---|
| 20–29 | 9.3–26.5 | Peak reproductive years |
| 30–39 | 8.7–25.1 | Gradual decline begins |
| 40–49 | 6.8–21.5 | Clinically meaningful decline |
| 50–59 | 7.2–24.0 | Wide range; varies substantially |
| 60 and above | 6.6–18.1 | Lower expected range |
pg/mL to ng/dL conversion: divide by 10. So 15 pg/mL = 1.5 ng/dL. Some labs report in ng/dL; the numbers will appear 10x smaller.
pg/mL to pmol/L conversion: multiply by 3.47. So 15 pg/mL ≈ 52 pmol/L.
Why ranges vary between labs: LabCorp (test #140103) and Quest Diagnostics use different assay platforms and have established their reference ranges from different population studies. Results from one lab should not be compared directly to reference ranges from another. If you switch labs, your trend comparison requires caution.
Quest Diagnostics reports free testosterone direct under test code 15983 with slightly different reference ranges. Always use the reference range printed on your specific report.
What Does Low Free Testosterone (Direct) Mean?
Here is what it means clinically:
A low result means less biologically active testosterone is available to your tissues than expected for your age. This can occur through two distinct mechanisms:
1. Low total testosterone (primary or secondary hypogonadism) If both total and free testosterone are low, the testes are not producing enough testosterone overall. This may be primary (testicular failure — the testes themselves are not functioning normally) or secondary (the pituitary is not sending adequate stimulation signals — LH and FSH are low or inappropriately normal).
2. Low free testosterone with normal total testosterone This is a clinically distinct and often overlooked pattern. If total testosterone is normal but free testosterone is low, the likely explanation is elevated SHBG — which is binding more testosterone than usual, leaving less in the free, active fraction. Causes of elevated SHBG include aging, liver disease, hyperthyroidism, certain medications, and low insulin levels. This pattern is especially common in older men and in men with metabolic or liver conditions.
Causes of low free testosterone (all mechanisms):
- Elevated SHBG (most common cause of low free with normal total)
- Primary hypogonadism — testicular failure (injury, mumps orchitis, Klinefelter syndrome, chemotherapy)
- Secondary hypogonadism — pituitary or hypothalamic dysfunction (including hyperprolactinemia, pituitary tumors, opioid use)
- Obesity — excess body fat increases aromatase activity, converting testosterone to estrogen
- Type 2 diabetes and insulin resistance — associated with reduced testosterone production
- Chronic illness — kidney disease, liver disease, HIV
- Medications — corticosteroids, opioids, some antidepressants, anti-androgens, GnRH agonists
- Sleep deprivation — testosterone is predominantly secreted during sleep; chronic poor sleep reduces levels
- Aging — testosterone production declines approximately 1–2% per year after age 30
Symptoms of Low Free Testosterone in Men
Symptoms overlap significantly with other conditions, which is why diagnosis requires both a low measured level and consistent symptoms:
- Sexual function: reduced libido, erectile dysfunction, reduced spontaneous erections, decreased ejaculatory volume
- Body composition: loss of muscle mass and strength, increased body fat (particularly visceral fat), difficulty maintaining fitness gains
- Energy and mood: fatigue, reduced motivation, depressed mood, irritability, reduced sense of well-being
- Cognition: difficulty concentrating, reduced mental sharpness
- Physical: reduced body and facial hair, hot flashes, decreased bone density (increased fracture risk with prolonged deficiency), reduced testicular size
- Sleep: worsened sleep quality, increased risk of sleep apnea
Important: these symptoms are non-specific. Fatigue, low libido, and mood changes have many potential causes. Low free testosterone is one possible explanation — but thyroid disorders, depression, sleep disorders, cardiovascular disease, and other conditions produce identical symptoms. A clinical diagnosis requires both a consistently low measured level (typically on two separate morning blood draws) and symptoms that are consistent with hypogonadism.
Low Free Testosterone with Normal Total Testosterone: What Does It Mean?
This is one of the most clinically important patterns in testosterone interpretation and one of the most searched scenarios.
When total testosterone is in the normal range but free testosterone is low, the most common explanation is elevated SHBG. SHBG binds testosterone tightly — higher SHBG means more of your total testosterone is locked up and unavailable to your cells.
This matters because: a man with total testosterone of 500 ng/dL (normal) and high SHBG could have a free testosterone level of 6 pg/mL (low for his age) — meaning his tissues are receiving less testosterone signal than the total number suggests.
Who gets this pattern:
- Older men (SHBG naturally rises with age)
- Men with liver disease or cirrhosis (the liver produces SHBG; liver dysfunction can raise or lower it)
- Men with hyperthyroidism
- Men taking certain medications including anticonvulsants and some antidepressants
- Men who are lean with low insulin levels (insulin suppresses SHBG)
What to do: if your total testosterone is normal but free testosterone is low, ask your doctor to check SHBG. The full picture requires: total testosterone + free testosterone (direct or calculated) + SHBG + LH + FSH + albumin. This panel distinguishes between the different causes of low free testosterone and guides what to do next.
What Does High Free Testosterone (Direct) Mean in Men?
Elevated free testosterone in men is less common than low and is often overlooked. High free testosterone can occur from:
- Exogenous testosterone use — testosterone replacement therapy, anabolic steroid use, or testosterone gels/injections producing supraphysiologic levels
- Testicular or adrenal tumors — rare causes of excess androgen production
- Congenital adrenal hyperplasia — genetic condition causing excess adrenal androgen production
- Low SHBG — if total testosterone is normal but SHBG is low, the free fraction will be elevated even without excess production. Causes of low SHBG include obesity, insulin resistance, hypothyroidism, and high-dose androgens.
Signs and symptoms of high free testosterone in men: acne, oily skin, aggressive mood, elevated red blood cell count (erythrocytosis), increased risk of blood clots, sleep apnea, potential worsening of benign prostatic hyperplasia, and — with prolonged elevation — testicular atrophy from suppressed LH signaling.
Free Testosterone by Age: What Is "Normal" vs. "Optimal"?
The reference ranges above represent population-based normative data — they define what is statistically common, not necessarily what is functionally ideal. Some clinicians and patients distinguish between:
Reference range normal — within the statistical distribution of the population tested by the lab. This is what your lab report uses.
Functional or optimal range — a level at which symptoms are absent and physiological function is maintained. Some endocrinologists and men's health specialists use 9–15 pg/mL as a minimum functional threshold, though this is not standardized in guidelines.
Important: the Endocrine Society guidelines for hypogonadism diagnosis do not specify a universal free testosterone threshold. They recommend that total testosterone below 300 ng/dL on two morning measurements is the threshold for considering treatment — and that free testosterone should be measured when total testosterone is borderline or when SHBG is suspected to be abnormal. "Optimal" free testosterone framing is more common in functional and integrative medicine than in mainstream endocrinology guidelines, and the evidence base for treating based on free testosterone alone (without total testosterone being low) is limited.
How Is Free Testosterone (Direct) Tested?
When is it ordered:
- When total testosterone is borderline low and SHBG is suspected to be elevated
- When symptoms of hypogonadism are present despite normal total testosterone
- When monitoring testosterone replacement therapy
- As part of a comprehensive male hormone panel
Timing: testosterone levels follow a circadian rhythm — levels are highest in the early morning (7–10 AM) and decline throughout the day. Most guidelines recommend collecting the sample between 7–10 AM. A result drawn in the afternoon may be 20–30% lower than a morning value and can create a false impression of deficiency.
LabCorp test details: Free Testosterone, Direct — test code 140103. Methodology: direct analog immunoassay. Note: equilibrium dialysis (test code 143575 at LabCorp) is the gold-standard method and may be ordered if the direct analog assay is felt to be unreliable for a specific clinical situation.
Quest Diagnostics: Free Testosterone, Direct — test code 15983.
Testosterone Replacement Therapy: When Is It Appropriate?
Low free testosterone alone does not automatically indicate treatment. The Endocrine Society recommends testosterone therapy only when:
- Total testosterone is consistently below the lower limit of normal on two separate morning measurements
- The patient has symptoms consistent with hypogonadism
- The underlying cause has been evaluated (and reversible causes addressed first)
Reversible causes worth addressing before starting TRT:
- Weight loss in obese men (reduces aromatase activity, raises free testosterone)
- Sleep improvement (testosterone is produced during sleep)
- Treatment of underlying hypothyroidism or diabetes
- Medication review and adjustment
Contraindications to testosterone therapy per Endocrine Society guidelines:
- Prostate or breast cancer
- Elevated PSA without evaluated cause
- Hematocrit above 50% (erythrocytosis)
- Untreated severe sleep apnea
- Severe lower urinary tract symptoms
- Recent cardiovascular event (within 6 months)
- Desire for near-term fertility (testosterone suppresses sperm production)
Monitoring on TRT: free testosterone and total testosterone should be rechecked 3–6 months after starting therapy to confirm levels are in the target range. Hematocrit, PSA, and lipids are also monitored.
Unit Conversion: pg/mL, ng/dL, and pmol/L
Free testosterone results may be reported in different units depending on the lab and country:
| To convert | Multiply by |
|---|---|
| pg/mL → ng/dL | ÷ 10 (15 pg/mL = 1.5 ng/dL) |
| ng/dL → pg/mL | × 10 |
| pg/mL → pmol/L | × 3.47 (15 pg/mL ≈ 52 pmol/L) |
| pmol/L → pg/mL | ÷ 3.47 |
| nmol/L (total T) → ng/dL | × 28.84 |
All the reference ranges on this page are in pg/mL. If your report shows ng/dL, multiply by 10 to compare.
FAQ about Free Testosterone, Direct (Male)
-
What is free testosterone (direct)?
It is a blood test that measures the small fraction of testosterone that is not bound to proteins — the portion immediately available for your body to use. "Direct" means the free fraction was physically measured in the lab, rather than calculated from a formula. -
What is a normal free testosterone level for men in pg/mL?
Using LabCorp direct assay reference ranges: 9.3–26.5 pg/mL for men aged 20–29; 8.7–25.1 pg/mL for men aged 30–39; 6.8–21.5 pg/mL for ages 40–49; 7.2–24.0 pg/mL for ages 50–59; and 6.6–18.1 pg/mL for men over 60. Always compare to your lab's specific reference range. -
What does low free testosterone direct mean?
It means the biologically active fraction of testosterone in your blood is below the expected range for your age. This can reflect low overall testosterone production, or normal total testosterone with elevated SHBG binding more of it. The clinical significance depends on whether symptoms are present and whether total testosterone and SHBG have been evaluated. -
What does it mean to have low free testosterone but normal total testosterone?
This usually indicates elevated SHBG — more testosterone than normal is being bound to this protein, leaving less in the free, active fraction. It is a distinct clinical pattern that requires SHBG testing and evaluation separate from straightforward low testosterone. -
What is free testosterone direct LC on my lab report?
"Free testosterone direct LC" refers to the LabCorp (LC) version of the free testosterone direct assay. Test code 140103. The reference ranges are the age-stratified values listed in the table above. -
What is the difference between free testosterone direct and calculated free testosterone?
Direct measures the free fraction using an immunoassay on your actual blood sample. Calculated estimates the free fraction using a mathematical formula (typically the Vermeulen equation) based on total testosterone, SHBG, and albumin. Calculated free testosterone using a validated formula is considered by many endocrinologists to be more reliable than the direct analog immunoassay, though both are used clinically. -
What is a good free testosterone level for a man?
"Good" depends on age. For men aged 30–50, most clinicians would consider levels above 9–10 pg/mL reassuring and levels below 7 pg/mL concerning if symptoms are present. These thresholds are not universal guidelines — they reflect general clinical practice patterns. A result should always be interpreted alongside symptoms and the full hormone panel. -
Can free testosterone be low even if total testosterone is normal?
Yes — and this is clinically important. Elevated SHBG is the most common reason. Men with high SHBG have more of their total testosterone locked up in a bound, inactive form, leaving less as free testosterone even when the total appears normal. -
What are the symptoms of low free testosterone in men?
The most common are reduced libido, erectile dysfunction, fatigue, loss of muscle mass, increased body fat, depressed mood, and reduced motivation. These are non-specific — many other conditions cause identical symptoms — so a consistently low measured level is required alongside symptoms for a clinical diagnosis. -
Should free testosterone be tested in the morning?
Yes — testosterone levels follow a circadian rhythm and are highest between 7–10 AM. Afternoon testing can produce values 20–30% lower than morning values, which can falsely suggest deficiency. Most guidelines recommend morning collection for accurate results. -
What does "free testosterone(direct) low" mean on my HealthMatters dashboard?
It means your result falls below the age-matched reference range for the direct assay method. The dashboard uses your lab's reference range (typically LabCorp or Quest) to flag values as low. This warrants discussion with your doctor, particularly if you have symptoms consistent with low testosterone.
Lab Results Explained and Tracked
What does it mean if your Free Testosterone, Direct (Male) result is too high?
Elevated free testosterone means more unbound, biologically active testosterone is circulating than expected for your age. In men, this is less commonly a spontaneous finding than low free testosterone, and it usually has an identifiable cause.
Common causes of high free testosterone in men:
The most important thing to establish first is whether the elevation is driven by excess testosterone production or by low SHBG. If SHBG is low — which occurs with obesity, insulin resistance, hypothyroidism, and high-dose androgen use — total testosterone can appear normal while free testosterone is elevated, because less is being bound. This is a different clinical picture from true excess production.
True excess production causes include exogenous testosterone use (testosterone replacement therapy producing supraphysiologic levels, anabolic steroids, testosterone gels or injections), testicular or adrenal tumors (rare), and congenital adrenal hyperplasia.
Effects of persistently high free testosterone in men:
- Acne and oily skin
- Aggressive mood and behavioral changes
- Elevated red blood cell count (erythrocytosis) — increases clotting risk
- Sleep apnea or worsening of existing sleep apnea
- Elevated blood pressure
- Gynecomastia — some excess testosterone converts to estrogen via aromatase
- Testicular atrophy — high exogenous testosterone suppresses LH, reducing the testes' own production signal
- Potential worsening of benign prostatic hyperplasia
- Dyslipidemia — adverse effects on cholesterol in some cases
A single elevated result warrants repeat testing alongside total testosterone, SHBG, LH, FSH, and PSA to determine the mechanism and appropriate next steps. An isolated elevation on a direct analog immunoassay should be confirmed — the direct assay has known variability and a single result can reflect measurement error.
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What does it mean if your Free Testosterone, Direct (Male) result is too low?
A low free testosterone result means the biologically active fraction of testosterone in your blood is below the expected range for your age. This is the portion your tissues actually use — so when it falls, the effects are felt throughout the body even if total testosterone appears borderline normal.
Why free testosterone specifically can be low:
The most common reason for low free testosterone with a normal total testosterone result is elevated SHBG — the binding protein that locks testosterone out of circulation. When SHBG is high, more of your total testosterone is bound and unavailable, leaving a smaller free fraction. This is especially common in older men, men with liver or thyroid conditions, and men on certain medications. It means the cause of your low result may not be the testes at all.
When both free and total testosterone are low, the testes are not producing enough testosterone overall — either because the testes themselves are failing (primary hypogonadism) or because the pituitary is not sending adequate signals to stimulate production (secondary hypogonadism).
Common causes:
- Elevated SHBG — aging, liver disease, hyperthyroidism, certain medications
- Obesity — excess body fat increases conversion of testosterone to estrogen via aromatase
- Type 2 diabetes and insulin resistance
- Chronic illness — kidney disease, HIV, inflammatory conditions
- Medications — opioids, corticosteroids, some antidepressants, anti-androgens
- Sleep deprivation — testosterone is primarily secreted during sleep
- Pituitary or hypothalamic dysfunction including hyperprolactinemia
- Testicular injury, infection, or prior chemotherapy or radiation
Symptoms associated with low free testosterone in men:
- Reduced libido and sexual interest
- Erectile dysfunction or reduced spontaneous erections
- Fatigue and reduced motivation
- Loss of muscle mass and strength, increased body fat
- Depressed mood, irritability, reduced sense of well-being
- Difficulty concentrating
- Reduced body and facial hair
- Reduced bone density — increasing fracture risk over time
- Enlarged breast tissue (gynecomastia) in some cases
These symptoms are non-specific — many other conditions produce identical effects. A clinical diagnosis requires both a consistently low measured level on two separate morning blood draws and symptoms consistent with hypogonadism.
Low free testosterone does not automatically mean testosterone therapy. Before starting treatment, your doctor will look for reversible causes — weight loss in overweight men often raises free testosterone substantially; addressing sleep disorders, thyroid dysfunction, or causative medications may be sufficient. The Endocrine Society recommends against testosterone therapy in men who have prostate or breast cancer, elevated PSA without a clear cause, hematocrit above 50%, untreated severe sleep apnea, a near-term plan for fatherhood, severe lower urinary tract symptoms, poorly controlled heart failure, a recent heart attack or stroke within six months, or a tendency to form blood clots. Treatment is appropriate when levels are consistently low, symptoms are present, reversible causes have been addressed, and contraindications are absent.
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