5-OH-indoleacetic Acid (5-HIAA) is a downstream metabolite of serotonin, which is formed from the essential amino acid tryptophan. Most blood serotonin and urinary 5-HIAA comes from serotonin formation outside of the CNS, primarily the liver and enterochromaffin cells in the gastrointestinal tract. Serotonin is further metabolized by monoamine oxidase to become 5-HIAA.

5-OH-indoleacetic Acid is a breakdown product of serotonin that is excreted in the urine. Serotonin is a hormone found at high levels in many body tissues. Serotonin and 5-OH-indoleacetic Acid are produced in excess amounts by carcinoid tumors, and levels of these substances may be measured in the urine to test for carcinoid tumors.

Pyroglutamate (or 5-Oxoproline) is an intermediate in the glutathione metabolism and a marker of glutathione deficiency.

Pregnenetriol is a metabolite of 17a-pregnenolone, an intermediary resulting from the hydroxylation of pregnenolone by CYP17A1 enzyme.

Two types of antibodies are detected in the Western blot test.

This particular marker is called 58 KD (IGG) Band and hence is a IgG antibody marker. IgG antibodies are a sign of an older infection. In contrast, IgM antibodies reflect a relatively recent infection. 

IgM antibodies usually disappear after eight weeks post-exposure. 
IgG remains in the serum for a very long time. 

5a-Androstanediol is a testosterone metabolite that is more androgenic than b-Androstanediol. It is metabolized via the 5-alpha metabolic pathway (= increased 5α-reductase activity). In contrast, the 5-beta metabolism makes androgens less potent.

Increased 5a-reductase activity may be accompanied by clinical signs of androgenicity (excess facial hair growth, scalp hair loss, acne, irritability, oily skin, prostate issues in men...etc).

5A-AD is a metabolite of 5αDHT.

Research suggests:

- that postmenopausal women may experience low levels of this metabolite.

- elevations of this pathway in females maybe due to PCOS and hirsutism.

5a-DHT is a testosterone metabolite. 

5a-DHT is essential for the development of the male sex characteristics before birth, particularly the formation of the external genitalia. In the adult, 5a-DHT is needed to develop and maintain male gender characteristics, such as facial hair, deep voice, and muscle growth. In women, 5a-DHT may induce the onset of puberty and causes the body and pubic hair growth.

Increased 5a-DHT levels are found in about 40% of patients with idiopathic hirsutism and 35% of patients with polycystic ovarian syndrome (=PCOS).

5a-DHT is a testosterone metabolite. 

5a-DHT is essential for the development of the male sex characteristics before birth, particularly the formation of the external genitalia. In the adult, 5a-DHT is needed to develop and maintain male gender characteristics, such as facial hair, deep voice, and muscle growth. In women, 5a-DHT may induce the onset of puberty and causes the body and pubic hair growth.

Increased 5a-DHT levels are found in about 40% of patients with idiopathic hirsutism and 35% of patients with polycystic ovarian syndrome (=PCOS).

5a-Dihydrotestosterone (5a-DHT), a potent androgen, is a crucial marker in the ZRT Laboratory Urinary Neurotransmitters panel, underscoring its significance in male health and hormonal research. 5a-DHT, derived from testosterone via the enzyme 5-alpha reductase, plays a key role in male physiology, including the development of male characteristics, maintenance of prostate health, and regulation of hair growth.

5a-DHT is a testosterone metabolite. 

5a-DHT is essential for the development of the male sex characteristics before birth, particularly the formation of the external genitalia. In the adult, 5a-DHT is needed to develop and maintain male gender characteristics, such as facial hair, deep voice, and muscle growth. In women, 5a-DHT may induce the onset of puberty and causes the body and pubic hair growth.

Increased 5a-DHT levels are found in about 40% of patients with idiopathic hirsutism and 35% of patients with polycystic ovarian syndrome (=PCOS).

5A-DHT is converted from testosterone by 5-α reductase in the ovaries and peripherally in fat tissue.